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John FowlerAssociate ProfessorPh.D., 1994, University of California, Berkeleyfowlerj@science.oregonstate.eduFaculty of Center for Genome Research and Biocomputing
Office/Lab: Cordley 4071/4066 |
Plant cell biology and development.
Primary research interests are in the molecular mechanisms that govern the establishment, maintenance and consequences of cell polarity. Despite recent advances in our understanding of cell polarity, gained primarily from Saccharomyces cerevisiae, such mechanisms are largely uncharacterized in plants. We are currently using a variety of complementary techniques in cell biology, molecular biology and genetics to address the role of small GTPases in these processes in two experimentally tractable plants: the brown alga Fucus distichus, and the monocot Zea mays.
Images of plasmolyzed maize leaf cells, illustrating plasma membrane attachment to the surrounding cell wall. Fluorescent images are of a Green Fluorescent Protein fusion that has been targeted to the cell's plasma membrane by a unique signal present in the maize ROP7 protein.
Rex Cole
Zuzana Vejlupkova
Kory Herrick
Malem Gutema
Jessica McElravy
MCB 553 Structure and Function of Cells
MCB 556 Cell Signaling and Development
BI 102 Genetics, Evolution and Behavior
Fowler, J.E., Vejlupkova, Z., Goodner, B.W., Lu, G. and Quatrano, R.S. (2004) Localization to the rhizoid tip implicates a Fucus distichus Rho family GTPase in a conserved cell polarity pathway. Planta (In press)
Arthur, K.M., Vejlupkova, Z., Meeley, R.B. and Fowler, J.E. (2003) The maize ROP2 GTPase provides a competitive advantage to the male gametophyte. Genetics, 165: 2137-2151. http://www.genetics.org/cgi/content/full/165/4/2137
Christensen, T.M., Vejlupkova, Z., Sharma, Y.K., Arthur, K.M., Spatafora, J.W., Albright, C., Duvick, J.P., Quatrano, R.S. and Fowler, J.E. (2003) Conserved subgroups and developmental regulation in the monocot rop gene family. Plant Physiology, 133:1791-1808. http://www.plantphysiol.org/cgi/content/full/133/4/1791
Fowler, J.E. (2003) A gametophyte factor on chromosome 9 affects both male and female gametophytes, Maize Genetics Cooperation Newsletter 77: 26-27. http://www.maizegdb.org/mnl/77/59fowler.html
Ivanchenko, M., Vejlupkova, Z., Quatrano, R.S. and Fowler, J.E. (2000). Maize ROP7 GTPase contains a unique, CaaX box-independent plasma membrane targeting signal. Plant J. 24: 79-90.
Fowler, J.E. Cell polarity in algae and vascular plants. (2000). In Frontiers in Molecular Biology: Cell Polarity, Drubin, D. (ed), pp. 141 - 180. Oxford University Press, New York
*Muehlbauer, G.J., *Fowler, J.E., Girard, L., Tyers, R., Harper, L. and Freeling, M. (1999). Ectopic expression of the maize homeobox gene liguleless3 alters cell fates in the leaf. Plant Phys. 119:651-62.
Muehlbauer, G.J., Fowler, J.E. and Freeling, M. (1997). Sectors expressing the homeobox gene
liguleless3 implicate a time-dependent mechanism for cell fate acquisition along the proximal-distal axis of the maize leaf. Development 124: 5097-5106.
Fowler, J.E. and Quatrano, R.S. (1997). Plant cell morphogenesis: plasma membrane interactions with the cytoskeleton and cell wall. Annu. Rev. Cell Dev. Biology 13: 697- 743.
Fowler, J.E. and M. Freeling. 1996. Genetic analysis of mutations that alter cell fates in maize leaves: dominant Liguleless mutations. Developmental Genetics 18:198-222
Fowler, J.E., G.J. Muehlbauer, and M. Freeling. 1996. Mosaic analysis of the Liguleless3 mutant phenotype in maize by coordinate suppression of Mutator-insertion alleles. Genetics 143:489-503
Fowler, J.E. and R.S. Quatrano. 1995. Cell polarity, asymmetric division, and cell fate determination in brown algal zygotes. Seminars in Developmental Biology 6:347-358.
