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microfluidics [2014/09/24 11:16]
ethanminot
microfluidics [2014/09/26 12:41] (current)
ethanminot
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 ===Laminar flow cell=== ===Laminar flow cell===
  
-Tal developed this flow cell during his PhD. It is closely modeled on a flow cell developed at TU Delft. ​Here is the basic operation:+Tal developed this flow cell during his PhD. It is closely modeled on a flow cell developed at TU Delft by K.M. Hooning. Hooning'​s thesis ​is available on the Tdrive. The sequence of images below shows blue dye and yellow being pumped through the flow cell. There is a sharp boundary between the two fluids. The boundary can be moved up and down by adjusting the relative pressure applied to the blue fluid and the yellow fluid.
  
 +{{::​screen_shot_2014-09-26_at_12.39.04_pm.png|}}
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 +Here is the nuts and bolts of operating the laminar flow cell:
 |{{::​flowcell1.jpg|}}|The chip sits on a standard glass slide. The slide makes it easy to fine-tune the position of the chip. The slide also provides electrical isolation from the metal block.| |{{::​flowcell1.jpg|}}|The chip sits on a standard glass slide. The slide makes it easy to fine-tune the position of the chip. The slide also provides electrical isolation from the metal block.|
 |{{::​flowcell2.jpg|}}|The plexiglass block is designed to feed liquid onto the chip. The interface between the block and chip requires a glass slide with three holes (the holes align with the three o-rings) and a [[http://​en.wikipedia.org/​wiki/​Gasket|gasket]]. The glass slide with 3 holes is first positioned and gently clamped in place (silver screws on the corners). Then a gasket is fabricated out of parafilm. The parafilm is cut with a scalpel. The inside of the gasket is just big enough to frame the three holes. The parafilm is gently pressed onto the glass. The parafilm adheres well. The flap of extra parafilm is sliced off, using the edge of the glass as a guide for the scalpel. | |{{::​flowcell2.jpg|}}|The plexiglass block is designed to feed liquid onto the chip. The interface between the block and chip requires a glass slide with three holes (the holes align with the three o-rings) and a [[http://​en.wikipedia.org/​wiki/​Gasket|gasket]]. The glass slide with 3 holes is first positioned and gently clamped in place (silver screws on the corners). Then a gasket is fabricated out of parafilm. The parafilm is cut with a scalpel. The inside of the gasket is just big enough to frame the three holes. The parafilm is gently pressed onto the glass. The parafilm adheres well. The flap of extra parafilm is sliced off, using the edge of the glass as a guide for the scalpel. |

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