Visualizing single fluorophores stuck to a glass slide

It should be feasible to image single fluorophores immobilized on a surface with a widefield microscope. You'll probably need decently large integration times, and won't be able to see them by eye (longer than the eye's 0.3 second integration). Making “test samples” with fluorophores deposited from dilute solutions is trickier than it sounds, due to things like molecules aggregating as solvent dries, staying in the liquid if soluble, etc., so it will take some trial and error. You will have to test several. Something in the low nanomolar range might be a good starting point… but it of course depends how well the dye sticks to the surface. Spin coater is probably a good idea.

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